基诺莱斯生物科技

基诺莱斯产品被《自然》杂志文章引用

意大利塔拉莫大学的Angelo Canciello等在2017年6月19日的《自然》杂志发表的《孕酮预防绵羊羊膜上皮细胞上皮-间质转化和提高他们的免疫调节特性》一文中，提供了孕酮（P4）预防绵羊羊膜上皮细胞（oAEC）上皮-间质转化（EMT）的分子学证据。在测定细胞因子TGF- β、IL-4和IL-10的表达时，他们采用了我基诺莱斯的相关ELISA试剂盒，并在文章中的两处做了引用注明。

具体引用情况如下：

“在标准条件下扩增的oAEC通过上调EMT转录因子自发获得间质特性， 通过抑制EMT诱导机制比如TGF- β的自分泌（定量测定采用基诺莱斯科技有限公司生产的Nori Sheep TGF-β3 ELISA试剂盒）和细胞内相关信号的活化， P4可以阻止这种表型的变化。从细胞培养基中去掉P4然后细胞传代一次，P4的这种作用仍然存在，并且P4可以很快逆转扩增中正在进行EMT转化的oAEC间质表型。此外，P4促进多能行基因的急性上调进而提高基底和LPS诱导的oAEC抗炎反应，表现为抗炎细胞因子表达增加（定量测定采用基诺莱斯科技有限公司生产的Nori Sheep IL-4 and IL-10 ELISA试剂盒）和促炎性细胞因子表达降低。综合上述结果，P4对于维持oAEC的上皮表型提高其生物学特性至关重要，因此，为了提高这种上皮干细胞的治疗潜力，我们提出了一种新的细胞培养方法。”

《自然》杂志在国际上影响力巨大，其影响因子高达38.138，我公司产品被其引用有重大的意义，这是一个小的科技公司成功的标志。首先有利于提升公司产品知名度，其次产品质量也将在国际上得到充分肯定，公司产品将会得到更好的推广和发展。

附件：原英文文献

Title: Progesterone prevents epithelialmesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties

Published at: Nature, 19-Jun-17, doi:10.1038/s41598-017-03908-1.

Authors: Angelo Canciello 1, Valentina Russo1, Paolo Berardinelli1, Nicola Bernabò1, Aurelio Muttini1, Mauro Mattioli2 & Barbara Barboni1

1Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via Renato Balzarini 1, 64100, Teramo, Italy. 2Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) “G. Caporale”, Campo Boario, 64100, Teramo, Italy. Correspondence and requests for materials should be addressed to A.C. (acanciello@unite.it).

The in vitro expansion is detrimental to therapeutic applications of amniotic epithelial cells (AEC), an emerging source of fetal stem cells. This study provides molecular evidences of progesterone (P4) role in preventing epithelial-mesenchymal transition (EMT) in ovine AEC (oAEC). oAEC amplified under standard conditions spontaneously acquired mesenchymal properties through the up-regulation of EMT-transcription factors. P4 supplementation prevented phenotype shift by inhibiting the EMT inducing mechanism such as the autocrine production of TGF-β (quantified by Nori Sheep TGF-β3 ELISA Kit, Genorise Scientific, Inc.) and the activation of intracellular related signaling. The effect of P4 still persisted for one passage after steroid removal from culture as well as steroid supplementation promptly reversed mesenchymal phenotype in oAEC which have experienced EMT during amplification. Furthermore, P4 promoted an acute up-regulation of pluripotent genes whereas enhanced basal and LPS-induced oAEC anti-inflammatory response with an increase in anti-inflammatory (quantified by Nori Sheep IL-4 and IL-10 ELISA Kits, Genorise Scientific, Inc.) and a decrease in pro-inflammatory cytokines expression. Altogether, these results indicate that P4 supplementation is crucial to preserve epithelial phenotype and to enhance biological properties in expanded oAEC. Therefore, an innovative cultural approach is proposed in order to improve therapeutic potential of this promising source of epithelial stem cells.